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Infection and ResponseHighermonoclonal antibodiesdiagnostic testingcontrol

AQA GCSE · Question 04.7 · Infection and Response

Control areaAny free monoclonalantibodies bind to thisareaTest areaContains a chemical thatbinds to the monoclonalantibody-steroid complexEnd of test stripContains free monoclonalantibodies

Figure 5 shows a test strip that can detect the presence of an anabolic steroid in an athlete's urine. The end of the test strip is dipped in urine. The urine moves up through the test strip. The test area and the control area contain a dye. The dye turns blue when monoclonal antibodies bind to it. Suggest the purpose of the control area in the test strip.

How to approach this question

In any test, a "control" is used to check something. What needs to be checked for this test to be considered valid? Think about what would happen if the liquid didn't move up the strip properly.

Full Answer

The control area serves as a check to confirm that the test strip is functioning correctly. It contains immobilised antibodies that bind to any free monoclonal antibodies, regardless of whether they are bound to the steroid or not. If a blue line appears in the control area, it proves that: 1. The urine has flowed correctly up the strip. 2. The monoclonal antibodies have been successfully picked up by the urine and have travelled up the strip. If the control line does not appear, the test is invalid and the result cannot be trusted, even if the test line is also blank.

Common mistakes

✗ Saying it is to compare the colours (it's a check, not a comparison). ✗ Saying it tests for something else in the urine. Its purpose is to validate the test procedure itself.

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